We performed GO and KEGG analysis of GSE15227 database to select the differential genes CHST3 and CSPG4 in grade II, III and IV intervertebral disc degeneration, IHC and WB to detect the protein profile of CHST3 and CSPG4, Co‐IP for the interaction between CHST3 and CSPG4. Here, AQP1 is linked to Intervertebral disk degeneration.