The isolated CESCs from three patients of grade II, III and IV groups with intervertebral disc degeneration were applied to detect the level of CD73, CD90 and CD105 by flow cytometry, and the results demonstrated that compared with grade II group, the expression level of these proteins were obviously decreased to a large extent (P < .05) (Figure 3B). This evidence concerns the gene NT5E and Intervertebral disk degeneration.