Dual-luciferase reporter assay showed a decrease in luciferase activity in CRC cells cotransfected with miR-194-5p-mimics and SOX2OT 3′-UTR-WT while there was an increase in luciferase activity in CRC cells cotransfected with miR-194-5p-inhibitor and SOX2OT 3′-UTR-WT compared with that in HT29 cells cotransfected with miR-NC and SOX2OT 3′-UTR-WT (Fig. 4D), suggesting that miR-194-5p was a direct bind site of SOX2OT in CRC. The gene discussed is SOX2-OT; the disease is colorectal carcinoma.