PNPLA2 and neoplasm: While ATGL-KO cells showed a higher consumption of glucose and a higher production of lactate (normalized to protein amount) as multicellular tumor spheroids, we found no significant difference in lactate production and glucose consumption when the same cells were cultured in 2D (supplemental Fig. S4, D and E), suggesting that the observed more glycolytic phenotype is really a characteristic of ATGL-KO spheroids.