MPZL3 and obesity due to melanocortin 4 receptor deficiency: To more extensively characterize the metabolic effects of Mpzl3 knockdown, a separate cohort of male C57BL/6N littermates were placed on HED for 7‐weeks to induce obesity (diet‐induced obese, DIO), followed by IP injection of Mpzl3 ASO (ASO 723571, 50 mg/kg, twice weekly).