To verify whether SHCBP1 is upregulated in gastric cancer and has clinical relevance with HER2 amplification, we performed IHC analysis of a tissue microarrays (TMAs), including 223 paired gastric cancerous and adjacent normal tissues, the results indicated that SHCBP1 was indeed highly expressed in gastric cancer samples (Fig. 2a, b). The gene discussed is ERBB2; the disease is gastric cancer.