ACAT1 and hepatocellular carcinoma: TIL isolated from resected HCC and stimulated overnight in the presence of an ACAT inhibitor showed significantly enhanced IFNγ+ TAA-specific responses (as a proportion of CD8+ T cells or of total live CD45+ TIL, Fig. 4a, b; Supplementary Fig. 4d), as well as the induction of de novo CD8+ T cell responses in some patients that had no detectable peptide-specific IFNγ production when untreated (Supplementary Fig. 4e), with no changes in the frequency of global CD3+ or CD8+ TIL (Supplementary Fig. 4f).