Being a derivative of LNCaP with its ability to metastasize to bone, LNCaP C4-2B is a PCa cell line that can induce bone lesions with mixed osteoblastic and osteolytic function.23,24 PC3 is a bone metastatic PCa cell line, which promotes high osteolytic lesions with minimal or no osteoblastic function.25,26 Osteoclast precursors were isolated from the bone marrow of C57BL6 mice (forelimbs and hind limbs) and were differentiated using RANKL and M-CSF (control condition) and conditioned medium (CM) from PC3 and LNCaP C4-2B cells separately. This evidence concerns the gene CSF1 and posterior cortical atrophy.