We respectively isolated PMNTUMORand PMNCTRfrom splenocytes of tumor-bearing and control mice by FCM and co-cultured them with splenocytes acquired from OT-1 mice with different ratio.Fig. 3Ashowed that PMNTUMORcould significantly inhibit the proliferation of OVA-activated CD8+T cells at ratio of 1:2 and 1:1, compared with PMNCTR. This evidence concerns the gene CD8A and neoplasm.