After that, we assessed the level of miR-326 in lung cancer cells that were transfected with si-PCAT6 or PCAT6 vector, and we found that the level of miR-326 was markedly increased in PCAT6 down-expressing H446 and H1975 cells or decreased in PCAT6 up-expressing H446 and H1975 cells (Figure 4E-F, P < 0.05). This evidence concerns the gene PCAT6 and lung carcinoma.