Mechanism analysis revealed that sevoflurane suppressed the Wnt5a expression in lung cancer cells by regulating PCAT6 sponging miR-326, suggesting that sevoflurane modulated PCAT6/miR-326/Wnt5a/β-catenin loops to restrain lung cancer process and compensated for the research gap in the anti-tumor mechanism of sevoflurane. Here, WNT5A is linked to lung cancer.