Furthermore, the risk score was applied in the TCGA RNA-seq cohort for ROC analysis to explore the sensitivity and specificity of the risk score compared with other covariates, providing the AUC of disease status (GBM vs. LGG, AUC = 0.6953), IDH1 mutation status (wild-type vs. mutant, AUC = 0.7711), MGMT methylation status (unmethylated vs. methylated, AUC=0.6488), and the risk score (as a continuous variable, AUC = 0.7490, Supplementary Figure S5). The gene discussed is MGMT; the disease is glioblastoma.