Here, we found that miR-28-5p level was significantly decreased in human DLBCL tissues, and silencing of miR-28-5p could effectively block the decreased cell malignant phenotype caused by TUC338 knockdown, suggesting that miR-28-5p also plays a tumor-inhibiting role in DLBCL. This evidence concerns the gene PCBP2-OT1 and diffuse large B-cell lymphoma.