In the present study, key genes responsible for the NF-κB activation were investigated, namely, CHUK, IKBKB, NFKBIA, NFKB1 and RELA. Stimulation with the cytokine combination in HaCaT cells led to elevation in all NF-κB-related genes, consistent with a role in inducing psoriasis-like inflammation. This evidence concerns the gene RELA and psoriasis.