The lag time observed in plasmin generation by HDMVEC-surface bound tPA, about 20–30 min, is similar to that described for cerebral microvascular endothelial cells (Semov et al., 2005), but much longer than for pancreatic or breast cancer cells (Diaz et al., 2004; Sharma et al., 2010). This evidence concerns the gene PLAT and breast carcinoma.