The Western blot results revealed that in MDCK cells, curcumin could downregulate B-Raf, upregulate Raf-1 and inhibit ERK signalings, indicating that curcumin inhibits cyst development via suppressing Ras/B-Raf/MEK/ERK signaling pathway (Gao et al., 2011). This evidence concerns the gene BRAF and cyst.