ANXA5 and urinary bladder carcinoma: Annexin V/7-amino-actinomycin D (7-AAD) staining revealed that the combination of gemcitabine and MK-8776 increased the number of apoptotic bladder cancer cells (Fig. 3a, Fig. S5), while western blotting demonstrated that the expression of cleaved poly[ADP-ribose]-polymerase (PARP) was enhanced compared to either drug alone (Fig. 3b, Fig. S6).