In multiple of our individual analyses, the findings suggest that an enhancer hotspot associated with the oncogene POU2AF1 may be under positive selection: the hotspot come up as a top result in the TFBS analysis; it contributes to the enrichment of cancer genes among enhancer hotspots; it has an above-median ΔCAF z-score; and based on contributions of mutational signatures, we do not believe that background processes caused these mutations. Here, POU2AF1 is linked to cancer.