With the aim to decipher whether DNA polymerase α acts in concert with DNA polymerases δ and/or ε in the replication of the viral DNA, we silenced the corresponding regulatory subunits POLD2 and POLE2/DPB2 by VIGS in N. benthamiana, and tested the capacity of TYLCV to replicate in local infection assays and to infect systemically. This evidence concerns the gene POLD2 and infection.