As shown in this present study, interference with HA metabolism by inhibiting HAS3, CD44 or the precursor of HA synthesis can block autophagy flux and further arrest the tumor cell cycle in G1 phase, due to blocking autophagy flux leads to the inhibition of late-stage autophagy, we suspected that the inhibition of glioma cell proliferation by blocking HA metabolism is achieved through the inhibition of autophagy rather than its over-activation. This evidence concerns the gene CD44 and glioma.