In this study, to examine if blood cancer EVs could also induce secretion of MMP9 and IL-6 from monocytes, EVs were isolated from a variety of malignant haematopoietic cells including leukaemia (HG3, I83, K562) and myeloma (U266) by differential centrifugation and iodixanol density flotation gradient (Fig 1). Here, IL6 is linked to hematopoietic and lymphoid system neoplasm.