We also verified by western blot analysis the accumulation of GFP and the phosphorylation of both PKR and eIF2α upon SINV-GFP infection of HCT116 KI-DICER and HEK293T cells (S4D Fig) and chose as optimal SINV-GFP condition of infection in HCT116 KI-DICER cells the MOI of 0.1 for 24 h. Here, EIF2A is linked to infection.