To verify the expression levels of the candidate genes in KRAS G12 mutated CRC, expression plasmids of KRAS variants, designated pKRAS-WT (wild-type), pKRAS-A (G12A), pKRAS-C (G12C), pKRAS-D (G12D), pKRAS-R (G12R), pKRAS-S (G12S), pKRAS-V (G12V), and pLacZ (control vector), were transfected into the human 293 embryonic kidney cells harboring KRAS wild-type. Here, KRAS is linked to colorectal carcinoma.