Effects on the activity of some CHD4 mutations have been examined (i) using Drosophila melanogaster CHD4 homolog dMi-2 as a model (55), (ii) by cryoelectron microscopy to study the structure of Homo sapiens CHD4 engaged with a nucleosome core particle (57), (iii) in functional studies of engineered cancer cells (56), and (iv) using bioinformatics functional annotation tools (SIFT, Polyphen-2, Mutation Assessor). This evidence concerns the gene CHD4 and cancer.