In this work, some issues are conspicuous 1) for only one dose of MAF (20 mg/kg) were used, it was far from efficient to evaluate the dose-efficacy relationship of this reagent; 2) the positive control was absent in this study; 3) NLRP3 inflammasome or NF-ĸB, which one is the main action pathway in MAF treating LPS-induced mastitis in mice? Here, NLRP3 is linked to mastitis.