Furthermore, counting in the percentage of the CD4+T-cells subpopulations to the total CD4+ T cells by flow cytometry on isolated PMNLs revealed that LGS significantly induced the naïve cell by 1.22 folds from pre-LGS (48.3±9.3%) to post-LGS (58.9±9.2%), p< 0.0001, and central memory (TCM) cells increased by 1.32 folds from pre-LGS (29.0±8.0%) to post-LGS (38.4±9.6%), p< 0.0001, but effective memory (TEM) cells decreased by 1.31 folds from pre-LGS (36.8±7.6%) to post-LGS (27.9±7.5%), p< 0.0001 among the cohort of class III obesity subjects, as shown in Figure 4C. Here, CD4 is linked to obesity due to melanocortin 4 receptor deficiency.