To test if the conversion of PrPC intoPrPSc and the formation of infectious prion could beinduced in cultured neuronal cells by virus infection, we used the neurotropic IAVstrain, IAV/WSN to infect mouse PrPC-overexpressing N2acells, termed N2aC24 cells11, at different multiplicities of infection(MOIs). The gene discussed is PRNP; the disease is infection.