In the present study, we aimed to develop and validate a MRM method targeting simultaneously the potential protein biomarkers for respiratory health (CC16, NF-κB and OPN) or for renal dysfunction (HSA) as well as potential adjusters of renal handling (RBP4, MYO and β2M) and to allow relative quantification in urine from a study cohort of young children. This evidence concerns the gene SPP1 and Abnormal renal physiology.