The present study revealed both RIPK1/RIPK3/MLKL and RIPK1/caspase-8/caspase-3 pathways were inhibited in TNBC tumor samples and AQP1-expressing cell lines, which is in agreement with our presumption that both RIPK1-dependent necroptosis and apoptosis are attenuated by AQP1. The gene discussed is MLKL; the disease is neoplasm.