We asked whether ATF4-dependent ASS1 induction was unique to THP1 AML cells, or extended to other tumors, using cancer cell lines established from liquid and solid tumors, including AML (HL60, MOLM-13, and OCI-AML3), acute promyelocytic leukemia (NB4), acute lymphocytic leukemia (RS4;11), and prostate (LNCaP), bladder (RT112), and cervical (HeLa) carcinomas. This evidence concerns the gene ASS1 and cancer.