Among all enrolled subjects, serum CEA, AFP, and CA19-9 levels were measured in 2 out of 3 stage 0, 15 out of 19 stage I, 35 out of 38 stage II, 25 out of 28 stage III, 29 out of 37 stage IV CRC, 28 out of 35 AA, 20 out of 35 SP patients, and all control subjects. This evidence concerns the gene AFP and colorectal carcinoma.