Immunofluorescence images showed that the fluorescence of DD‐MCT was poor colocalization with TfR, whereas was good colocalized with GLUT1 in the glioma region, directly confirming that DD‐MCT could undergo a detachment from Tf‐TfR complex and subsequent MAN‐mediated targeting delivery once entering into brain parenchyma (Figure 3D). The gene discussed is SLC2A1; the disease is central nervous system cancer.