Double immunofluorescence staining of adipose differentiation-related protein (ADRP) and LC3 demonstrated an inverse relationship between ADRP positive areas and LC3 positive areas, as well as the colocalization of ADRP and LC3 on a part of small lipid droplets, suggesting that impaired autophagy increased hepatic steatosis and regulates lipid droplet turnover [36]. This evidence concerns the gene MAP1LC3A and fatty liver disease.