To explore the potential mechanism of circ-ZNF609, nuclear-cytoplasmic separation assay was performed and we found that circ-ZNF609 mainly existed in the cytoplasm of glioma cells, which meant that circ-ZNF609 might serve as a ceRNA to bind to miRNA, so as to regulate the expression of downstream genes. This evidence concerns the gene ZNF609 and central nervous system cancer.