Consistent with the above findings, Western blotting was used to detect changes in the protein levels of p-EGFR, EGFR, p-PI3K, PI3K p-AKT, AKT, p-FOXO3a, FOXO3a, p27 and p21 in subcutaneous tumor tissue and HepG2 cells treated with Maytenus compound. This evidence concerns the gene FOXO3 and neoplasm.