A second round of targeted NGS on subject IV-5 in this family identified a hemizygous c.706G>A (p. Glu236Lys) variant in the X-chromosome gene POU3F4 (NM_000307.5), a causative gene for X-linked nonsyndromic deafness DFNX2. The c.706G>A (p. Glu236Lys) variant segregated with the deafness in the five aforementioned male family members, but it was not detected in any other family members (Figures 3(a) and 3(b)). This evidence concerns the gene POU3F4 and X-linked nonsyndromic hearing loss.