HE staining and CD3 immunohistochemical staining showed that CD45RA depletion reduced the infiltration of T cells and histological GVHD scores in GVHD target organs and that additional CD276 depletion significantly further decreased lung-infiltrating T cells and histological GVHD scores in DR4-mismatched setting (Fig. 5a–c). The gene discussed is CD276; the disease is graft versus host disease.