To evaluate if the Zα2 domain is critical for ZBP1 to mediate tumor necroptosis during tumor development, we implanted MVT-1 CT, ZBP1 KO, and WT or different mutant ZBP1 reconstituted KO cells into the fat pads of mice and examined the status of MLKL phosphorylation and Casp-3 cleavage in the resulting tumors of these different cells. Here, CASP3 is linked to neoplasm.