When examining the levels of necroptosis of the tumors from these cells, we found that tumor necrosis is dramatically reduced in ZBP1 KO tumors compared to CRISPR CT tumors by comparing the middle sections of four different WT and ZBP1 KO tumors and the serial sections of three different pairs of WT and ZBP1 KO tumors (Fig. 3b and Supplementary Fig. 3d) and that, while there is abundant MLKL phosphorylation in necrotic areas of CRISPR CT tumors, little MLKL phosphorylation is detected in ZBP1 KO tumors (Fig. 3c). The gene discussed is MLKL; the disease is neoplasm.