ZBP1 and neoplasm: To examine whether ZBP1 is needed for tumor necroptosis during MVT-1 mammary tumor development, we generated CRISPR ZBP1 knockout (ZBP1 KO) and CRISPR control (CRISPR CT) MVT-1 cells by transient transfection and selected clones with no Cas9 expression for our study because Cas9 is immunogenic28.