Consistent with differences in the number of AgRP and GABAArc neurons, in CNO-injected AgRP-Cre mice, there was no obvious colocalization between c-Fos and AgRP neurons (Fig. 3b) and CNO reduced the majority of c-Fos expression in T1D Vgat-Cre mice (Fig. 3c), suggesting an effective action of CNO-induced neuron inhibition. This evidence concerns the gene SLC32A1 and type 1 diabetes mellitus.