IFNGR1 and melanoma: To identify metabolic regulators of macrophage function during the response to IFNγ within the TME, we FACS-isolated TAMs from WT and IFNγR−/− mice challenged with syngeneic B16f10 melanoma tumors (Supplementary Fig. 1A), performed RNA sequencing, then compared metabolic genes from the KEGG pathway dataset to data generated from primary mouse BMMs stimulated in vitro with LPS/IFNγ.