PTPRC and neoplasm: To evaluate the NRE1-dependent function of tumor infiltrating immune cells, and to identify which specific immune subsets are implicated in NRE1-dependent tumor control, CD45-positive cells were isolated from these tumors grown in WT and NRE1-KO mice by FACS, subjected to single-cell RNA sequencing (scRNAseq), then analyzed using the Seurat and in-house developed34,35 computational platforms.