Furthermore, patients with high RFC4 expression showed shorter overall and LN metastasis-free survival than those with low RFC4 expression and notably, patients with high levels of both RFC4 and nuclear NICD1 had significantly shorter LN metastasis-free survival times than those with high RFC4 or nuclear NICD1 alone (Fig. 7c and Supplementary Fig. 9b). This evidence concerns the gene RFC4 and lobular neoplasia.