CCND1 and neoplasm: Consistently, subcutaneous tumors formed by NICD1-mutant NSCLC cells presented significantly increased levels of RFC4, CCND1, and PCNA and a decreased proportion of TUNNEL-positive tumor cells as compared to those formed by vector-control cells, whereas subcutaneous tumors formed by RFC4-silenced NICD1-mutant NSCLC cells presented remarkable reductions in RFC4 protein levels and only marginally altered levels of NICD1, CCND1, or PCNA expression or apoptotic tumor cell proportion (Fig. 6d).