Similarly, silencing RFC4 reversed the activation of the Notch signaling pre-induced by NICD1 or JAG1 in NSCLC cells, and the Notch signaling in NSCLC cells pre-silenced with RFC4 became insensitive to stimulation of JAG1 overexpression in HUVECs (Supplementary Fig. 6e–g). The gene discussed is RFC4; the disease is non-small cell lung carcinoma.