RFC4 and non-small cell lung carcinoma: Additionally, while NSCLC cells expressing stabilized mutant NICD1 developed excessive lung metastases when they were injected intravenously and were able to form detectable tumors even when 5 × 103 cells were subcutaneously inoculated, silencing RFC4 could not compromise the potent metastatic and tumorigenic abilities of these NICD1-mutant NSCLC cells (Fig. 6c and Supplementary Fig. 8d).