Consistent with the previously recognized notion that Notch signaling activates directly or indirectly activates the expression of stemness- and metastasis-promoting genes46,47, our current study suggests that NSCLC cells with high levels of RFC4 and nuclear NICD1, which might constitute a positive signaling feedback loop, show potent metastasis and stemness properties and therefore might represent reasonable targets for the treatment of NSCLC metastasis and drug resistance. The gene discussed is RFC4; the disease is non-small cell lung carcinoma.