DI-1548 and DI-1859 are highly potent and effective in inhibition of the neddylation of cullin 3 in cells with different tissue types, such as osteosarcoma U2OS cell line (Fig. 6a), immortalized THLE2 liver cells (Fig. 6b), breast cancer MDA-MB-231 cell line (Supplementary Fig. 18), esophageal cancer KYSE70 cell line (Supplementary Fig. 19), and colon cancer HCT116 cell lines (Supplementary Fig. 20). The gene discussed is CUL3; the disease is breast carcinoma.