PMEL and melanoma: To confirm that our approach can be extended to tumor-associated antigens, mRBCs were conjugated with a different MHC I-peptide complex – H-2Db loaded with the gp100 peptide (a tumor antigen expressed on B16-F10 melanoma cells), plus 4-1BBL and IL-12 to generate mRBC-gp100-4-1BBL-IL-12.