Hence, to determine if glycoproteins can be delivered efficiently to the plasma membrane in AP4E1-, STX10-, and CHMP4C-depleted cells, the cell surface localization of glycoproteins gD and gE was examined by immunofluorescence of nonpermeabilized cells 12 h after infection in depleted and control siRNA-transfected cells. Here, CHMP4C is linked to infection.