Moreover, knock-down of LINC00649 downregulated YAP1 at both transcriptional and translational levels, and decreased the expression levels of EGFR, SOX2 and OCT4 to recover the normal functions of the Hippo pathway via releasing miR-16-5p in GC cells, suggesting that LINC00649 regulated the YAP1/Hippo signaling pathway by targeting miR-16-5p. Here, EGFR is linked to gastric cancer.