In RV myocardium from end-stage human PAH patients (Supplemental Table 2), we found increased expression collagen and ACh (Figure 3, A and B) with no significant differences in expression of α7 nAChR and ACh synthesis proteins (ChT, VAChT, and ChAT) (Figure 3C and Supplemental Figure 4). The gene discussed is CHAT; the disease is pulmonary arterial hypertension.