As 5-HT1A receptor (5-HT1AR) and brain-derived neurotrophicfactor (BDNF) are critically involved in depression and anxiety, wefurther evaluated 5-HT1A receptor functionality by [35S]GTPγS autoradiography and BDNF mRNA expression by in situ hybridization techniques. The gene discussed is HTR1A; the disease is depressive disorder.