However, in this case EGF also stimulated an additional pathway, the Rho/Rho kinase pathway, which was shown to partially contribute to the phosphorylation of Filamin A. Inhibitor studies highlighted that the RSKs were the predominant Filamin A kinases, but in the squamous carcinoma and colon cancer cells RSKs and Rho kinase co-operatively phosphorylate Filamin A [72]. The gene discussed is FLNA; the disease is colonic neoplasm.