To determine whether the increase in PD-L1 expression is tumor cell-intrinsic rather than a secondary signal from other cell types present in tumor tissues, we treated murine KP (393P and 344SQ) and human (H2122, H1299, H358, H157, and A549) lung cancer cell lines harboring varying co-mutations in vitro with AZD6244 and observed an increase in PD-L1 mRNA and protein expression in lung cancer cells following MEK inhibition (Fig. 1d, e; Supplementary Fig. 1a, b). This evidence concerns the gene CD274 and lung cancer.