We also integrated known ALS-associated risk genes with the TDP-43 interactome in GPiNs and show that the experiments in GPiNs recapitulate known interactions between TDP-43 and VCP, MATR3, FUS, ATXN2, HNRNPA2B1, and EWSR1 (P = 0.046, using a hypergeometric distribution; Fig. 2h). Here, ATXN2 is linked to amyotrophic lateral sclerosis.