To begin with, we determined AGAP2-AS1, miR-296, and NOTCH2 expression in lung cancer tissues and cell lines, and the results indicated that AGAP2-AS1 and NOTCH2 increased while miR-296 decreased in radioresistant lung cancer tissues and cells lines, respectively compared with radiosensitive tissues and normal lung epithelial cell BEAS-2B. Here, NOTCH2 is linked to lung cancer.