This study was performed to identify the role of exosomal AGAP2-AS1 in the progression of lung cancer via the miR-296/NOTCH2 axis, and we might speculate that macrophage-derived exosomal AGAP2-AS1 may influence the immunologic functions of lung cancer patients after radiotherapy with the involvement of miR-296/NOTCH2 axis. Here, AGAP2 is linked to lung cancer.