In conclusion, the prepared CD63-IMB could effectively isolate serum exosomes, significantly up-regulated expressions of FRlnc1 were found in cells, tumor tissues, serum and serum exosomes of GC patients, and the high expression of FRlnc1 in exosomes was suggested to be significantly correlated to LNM and TNM stage. This evidence concerns the gene CD63 and gastric cancer.